recombinant human soluble tweak  (MedChemExpress)

Journal: bioRxiv

Article Title: Hypoalbuminemia Increases Fibrin Clot Density and Impairs Fibrinolysis

doi: 10.1101/2025.02.03.635731

Figure Lengend Snippet: ( A , B ) Representative residual clots at the clot lysis assay endpoint (60 minutes) from pDTR -nephrotic rat plasmas supplemented with recombinant Albumin ( A ) or Ovalbumin ( B ) ( shows representative residual clots of unmanipulated samples). ( C ) Lysis, expressed as a percentage of rat PNP values, is corrected by rAlbumin, but not Ovalbumin, supplementation. WT: wild-type; DT: diphtheria toxin; pDTR : podocin-promoter driven diphtheria toxin receptor transgene; rAlbumin: recombinant albumin; OvA: ovalbumin (note: OvA is a serpin-family, not an albumin-family, protein); n=5-8 rats/group; * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: In other experiments, CLA was performed with tissue-type plasminogen activator (tPA; 20 nM) or plasmin (1.2 μM) in lieu of uPA or after immunodepleting or supplementing specific proteins, as indicated – most notably, recombinant human albumin (expressed in P. pastoris ; Millipore Sigma).

Techniques: Lysis, Recombinant

Journal: bioRxiv

Article Title: Hypoalbuminemia Increases Fibrin Clot Density and Impairs Fibrinolysis

doi: 10.1101/2025.02.03.635731

Figure Lengend Snippet: ( A ) Representative photomicrographs of clots formed from 1 st and 4 th albumin IQR pDTR -rat plasma (as in ) with Ovalbumin or rAlbumin supplementation. ( B ) Fibrin area. ( C ) Fibrin network density. ( D ) Fibrin clot porosity. IQR: interquartile range; WT: wild-type; DT: diphtheria toxin; pDTR : podocin-promoter driven diphtheria toxin receptor transgene; rAlbumin: recombinant human albumin; OvA: ovalbumin (note: OvA is a serpin-family, not an albumin-family, protein); n=8 rats/group; * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: In other experiments, CLA was performed with tissue-type plasminogen activator (tPA; 20 nM) or plasmin (1.2 μM) in lieu of uPA or after immunodepleting or supplementing specific proteins, as indicated – most notably, recombinant human albumin (expressed in P. pastoris ; Millipore Sigma).

Techniques: Clinical Proteomics, Recombinant

Journal: bioRxiv

Article Title: Hypoalbuminemia Increases Fibrin Clot Density and Impairs Fibrinolysis

doi: 10.1101/2025.02.03.635731

Figure Lengend Snippet: ( A ) Representative residual clots at the clot lysis assay endpoint (60 minutes) from albumin knockout rat plasmas (as in ) without and with recombinant Albumin (rAlbumin) supplementation. ( B ) Lysis, expressed as a percentage of rat PNP values, was diminished in homozygous and heterozygous albumin knockout rat clots and was corrected by rAlbumin supplementation. ( C ) Representative photomicrographs of clots formed from albumin knockout rat plasmas without and with rAlbumin supplementation. ( D ) Fibrin area. ( E ) Fibrin network density. ( F ) Fibrin clot porosity. rAlbumin: recombinant albumin; n=5 rats/group; *** P <0.001.

Article Snippet: In other experiments, CLA was performed with tissue-type plasminogen activator (tPA; 20 nM) or plasmin (1.2 μM) in lieu of uPA or after immunodepleting or supplementing specific proteins, as indicated – most notably, recombinant human albumin (expressed in P. pastoris ; Millipore Sigma).

Techniques: Lysis, Knock-Out, Recombinant